Lactobacillus reuteri suppresses E. coli O157:H7 in bovine ruminal fluid: Toward a pre-slaughter strategy to improve food safety?

• DOI: 10.1371/journal.pone.0187229
• Publication Date: 2017-11-01T20:57:24Z
• Authors: Yolande Bertin; Chloé Habouzit; Lysiane Dunière; Marie Laurier; Alexandra Durand; David Duchez; Audrey Segura; Delphine Thévenot...; Federico Baruzzi; Frédérique Chauch...; Evelyne Forano
• Keywords: Lactobacillus reuteri

The bovine gastrointestinal tract (GIT) is the main reservoir for enterohaemorrhagic Escherichia coli (EHEC) responsible food-borne infections. Therefore, it crucial to develop strategies, such as EHEC suppression by antagonistic microorganisms, reduce survival in GIT of cattle and limit shedding food contamination. Most human-derived Lactobacillus reuteri strains produce hydroxypropionaldehyde (HPA), an antimicrobial compound, during anaerobic reduction glycerol. capacity L. LB1-7, a strain isolated from raw milk, HPA its activity against O157:H7 (FCH6) were evaluated rumen fluid (RF) under strict anaerobiosis. was totally suppressed when incubated RF inoculated with LB1-7 supplemented 80 mM glycerol (RF-Glyc80). addition or alone did not modify RF. Glycerol converted (up 14 mM) incubation RF-Glyc80, production appeared be suppression. bactericidal concentration required level produced depended on physiological ecological environments. In vitro experiments also showed that exposed had very limited growth rectal contents. However, exerted endogenous microbiota perturbed feedstuff degradation presence potential administration view application finishing beef at time slaughter discussed. Further vivo studies will important confirm efficiency supplementation ruminants.