Multiple effects of toxins isolated from Crotalus durissus terrificus on the hepatitis C virus life cycle

• DOI: 10.1371/journal.pone.0187857
• Publication Date: 2017-11-15T13:40:12Z
• Authors: Jacqueline Farinh...; Carina Machado Pe...; Cintia Bittar; Mariana Nogueira ...; Guilherme Rodrigu...; Suely da Silva; Adélia Cristina O...; Carsten Zothner; Mark Harris; Suely Vilela Sampaio; Victor Hugo Aquino; Paula Rahal; Ana Carolina Gome...
• Keywords: Viral life cycle

Hepatitis C virus (HCV) is one of the main causes liver disease and transplantation worldwide. Current therapy expensive, presents additional side effects viral resistance has been described. Therefore, studies for developing more efficient antivirals against HCV are needed. Compounds isolated from animal venoms have shown antiviral activity some viruses such as Dengue virus, Yellow fever Measles virus. In this study, we evaluated effect complex crotoxin (CX) its subunits crotapotin (CP) phospholipase A2 (PLA2-CB) venom Crotalus durissus terrificus on life cycle. Huh 7.5 cells were infected with HCVcc JFH-1 strain in presence or absence these toxins was titrated by focus formation units assay qPCR. Toxins added to at different time points depending stage cycle be evaluated. The results showed that treatment PLA2-CB inhibited entry replication but no release observed. CX reduced not replication. By treating CP, an observed release, only compound. Our data demonstrated multiple