Probiotics reduce repeated water avoidance stress-induced colonic microinflammation in Wistar rats in a sex-specific manner

• DOI: 10.1371/journal.pone.0188992
• Publication Date: 2017-12-15T13:46:50Z
• Authors: Ju Yup Lee; Nayoung Kim; Ryoung Hee Nam; Sung Hwa Sohn; Sun Min Lee; Daeun Choi; Hyuk Yoon; Yong Sung Kim; Hye Seung Lee; Dong Ho Lee
• Keywords: Barostat

The colonic response to stress is greater in female rats than male rats. aim of this study was evaluate the effect probiotics repeated water avoidance (rWAS)-induced microinflammation model Wistar a sex-specific manner. three groups (no-stress, WAS, and WAS with probiotics) were exposed r-WAS for 1 h daily 10 days, Lactobacillus farciminis administered by oral gavage days animals group. visceromotor (VMR) colorectal distension (CRD) assessed using barostat noninvasive manometry before after exposure. Immunohistochemistry mast cells real-time polymerase chain reaction (RT-PCR) detection mucosal cytokines performed distal colon tissue sacrificed. Significant reduction VMR CRD (visceral analgesia) observed at 60 mmHg group (P = 0.045), but not males. In addition, showed significantly lower cell count comparison 0.013), phenomenon mRNA levels interferon-γ (IFNR), tumor necrosis factor-α (TNFA), interleukin (IL) 6, IL17 higher IFNR, TNFA, IL6 decreased females who received (all P < 0.050). conclusion, rWAS induced A 10-day-long treatment L. an effective therapy rWAS-induced rates,