High association of Cryptosporidium spp. infection with colon adenocarcinoma in Lebanese patients

• DOI: 10.1371/journal.pone.0189422
• Publication Date: 2017-12-20T15:08:43Z
• Authors: Marwan Osman; Sadia Benamrouz; Karine Guyot; Martha Baydoun; Emilie Frealle; Magali Chabe; Nausicaa Gantois; Baptiste Delaire; Anne Goffard; Albert Aoun; Nawaf Jurdi; Fouad Dabboussi; Gael Even; Christian Slomianny; Pierre Gosset; Monzer Hamze; Colette Creusy; Eric Viscogliosi; Gabriela Certad
• Keywords: Adult; Male; 0301 basic medicine; Adolescent; Colon; [SDV]Life Sciences [q-bio]; Science; Biopsy; Cryptosporidiosis; Cryptosporidium; Adenocarcinoma; Young Adult; 03 medical and health sciences; Age Distribution; [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases; Cancer detection and diagnosis; Humans; Lebanon; [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology; Aged; Cryptosporidium parvum; Aged, 80 and over; [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology; Q; R; Middle Aged; Colorectal cancer; 3. Good health; [SDV] Life Sciences [q-bio]; [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology; Logistic Models; Case-Control Studies; Colonic Neoplasms; Multivariate Analysis; [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases; Medicine; Digestion; Female; [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology; Research Article

The association between Cryptosporidium and human colon cancer has been reported in different populations. However, this not well studied. In order to add new strong arguments for a probable link cryptosporidiosis cancer, the aim of study was determine prevalence identify species among Lebanese patients.Overall, 218 digestive biopsies were collected Tripoli, Lebanon, from three groups patients: (i) patients with recently diagnosed intraepithelial neoplasia/adenocarcinoma before any treatment (n = 72); (ii) stomach 21); (iii) without but persistent symptoms 125). DNA extraction performed paraffin-embedded tissue. presence parasite tissues confirmed by PCR, microscopic observation immunofluorescence analysis. We identified high rate (21%) colonic treatment. This significantly higher compared 7% neoplasia gastrointestinal (OR: 4, CI: 1.65-9.6, P 0.001). When comparison done against normal biopsies, risk infection increased 11-fold group adenocarcinoma 11.315, 1.44-89.02, 0.003).This is first Lebanon reporting cancer. These results show that strongly associated being maybe potential etiological agent disease.