Ocular and uteroplacental pathology in a macaque pregnancy with congenital Zika virus infection

• DOI: 10.1371/journal.pone.0190617
• Publication Date: 2018-01-30T18:39:51Z
• Authors: Emma L. Mohr; Lindsey N. Block; Christina M. Newman; Laurel M. Stewart; Michelle Koenig; Matthew Semler; Meghan E. Breitbach; Leandro B. C. Tei...; Xiankun Zeng; Andrea M. Weiler; Gabrielle L. Barry; Troy H. Thoong; Gregory J. Wiepz; Dawn M. Dudley; Heather A. Simmons; Andres Mejia; Terry K. Morgan; M. Shahriar Salamat; Sarah Kohn; Kathleen M. Antony; Matthew T. Aliota; Mariel S. Mohns; Jennifer M. Hayes; Nancy Schultz-Darken; Michele L. Schotzko; Eric Peterson; Saverio Capuano; Jorge E. Osorio; Shelby L. O’Connor; Thomas C. Friedrich; David H. O’Connor; Thaddeus G. Golos
• Keywords: Zika Virus; Rhesus macaque; Chorioamnionitis

Congenital Zika virus (ZIKV) infection impacts fetal development and pregnancy outcomes. We infected a pregnant rhesus macaque with Puerto Rican ZIKV isolate in the first trimester. The was complicated by preterm premature rupture of membranes (PPROM), intraamniotic bacterial demise 49 days post (gestational day 95). Significant pathology at maternal-fetal interface included acute chorioamnionitis, placental infarcts, leukocytoclastic vasculitis myometrial radial arteries. RNA disseminated throughout tissues maternal immune system necropsy, as assessed quantitative RT-PCR for viral RNA. Replicating identified tissues, uterus, spleen fluorescent situ hybridization replication intermediates. Fetal ocular choroidal coloboma, suspected anterior segment dysgenesis, dysplastic retina. This is report prolonged both following congenital macaque. PPROM followed severe visual have not been described previously. While this case during PPROM, role on outcome cannot be precisely defined, further nonhuman primate studies will determine if increased risk or other adverse outcomes are associated infection.