Genetic diversity and recombination of enterovirus G strains in Japanese pigs: High prevalence of strains carrying a papain-like cysteine protease sequence in the enterovirus G population

• DOI: 10.1371/journal.pone.0190819
• Publication Date: 2018-01-11T19:41:09Z
• Authors: Shinobu Tsuchiaka; Yuki Naoi; Ryo Imai; Tsuneyuki Masuda; Mika Ito; Masataka Akagami; Yoshinao Ouchi; Kazuo Ishii; Shoichi Sakaguchi; Tsutomu Omatsu; Yukie Katayama; Mami Oba; Junsuke Shirai; Yuki Satani; Yasuhiro Takashima; Yuji Taniguchi; Masaki Takasu; Hiroo Madarame; Fujiko Sunaga; Hiroshi Aoki; Shinji Makino; Tetsuya Mizutani; Makoto Nagai
• Keywords: Strain (injury); Nucleotide diversity; Molecular Epidemiology

To study the genetic diversity of enterovirus G (EV-G) among Japanese pigs, metagenomics sequencing was performed on fecal samples from pigs with or without diarrhea, collected between 2014 and 2016. Fifty-nine EV-G sequences, which were >5,000 nucleotides long, obtained. By complete VP1 sequence analysis, isolates classified into G1 (17 strains), G2 (four G3 (22 G4 (two G6 G9 (six G10 (five a new genotype (one strain). Remarkably, 16 one strain identified in diarrheic (23.5%; four strains) normal (76.5%; 13 possessed papain-like cysteine protease (PL-CP) sequence, recently found USA Belgium genome, at 2C–3A junction site. This paper presents first report high prevalence viruses carrying PL-CP population. Furthermore, possible inter- intragenotype recombination events strains, including G1-PL-CP strains. Our findings may advance understanding molecular epidemiology evolution EV-Gs.