An immuno-assay to quantify influenza virus hemagglutinin with correctly folded stalk domains in vaccine preparations

• DOI: 10.1371/journal.pone.0194830
• Publication Date: 2018-04-04T17:38:34Z
• Authors: Madhusudan Rajendran; Weina Sun; Phillip Comella; Raffael Nachbagauer; Teddy John Wohlbold; Fatima Amanat; Ericka Kirkpatrick; Peter Palese; Florian Krammer
• Keywords: Stalk

The standard method to quantify the hemagglutinin content of influenza virus vaccines is single radial immunodiffusion assay. This assay primarily relies on polyclonal antibodies against head domain hemagglutinin, which main target antigen vaccines. Novel vaccine candidates that redirect immune response towards evolutionary more conserved stalk, including chimeric and headless constructs, are highly dependent structural integrity protein present conformational epitopes for neutralizing antibodies. In this study, we describe a novel enzyme-linked immunosorbent allows quantifying amount with correctly folded stalk domains could be further developed into potency stalk-based