Oncolytic effect of wild-type Newcastle disease virus isolates in cancer cell lines in vitro and in vivo on xenograft model

• DOI: 10.1371/journal.pone.0195425
• Publication Date: 2018-04-05T21:31:44Z
• Authors: Kseniya S. Yurchenko; Peipei Zhou; Anna V. Kovner; Evgenii L. Zavjalov; Lidiya V. Shestop...; Alexander M. Shes...
• Keywords: Virotherapy; Newcastle Disease; HeLa

Oncolyic virotherapy is one of the modern experimental techniques to treat human cancers. Here we studied antitumor activity wild-type Newcastle disease virus (NDV) isolates from Russian migratory birds. We showed that NDV could selectively kill malignant cells without affecting healthy cells. evaluated oncolytic effect 44 in 4 histogenetically different cell lines (HCT116, HeLa, A549, MCF7). The safety was also tested normal peripheral blood mononuclear (PBMC) viability tumor after incubation with by MTT. All lines, except for PBMC primary cells, had degrees susceptibility infection. Seven strains highest activity, and some demonstrated selectivity lines. In vivo, described intratumoral NDV/Altai/pigeon/770/2011 against subcutaneous non-small lung carcinoma using xenograft SCID mice model. animals were responsive therapy. Histology confirmed therapy-induced destructive changes growing necrotic bulk density tissue. Our findings indicate no on suppresses growth mice.