The chloroplast genome sequence of bittersweet (Solanum dulcamara): Plastid genome structure evolution in Solanaceae

• DOI: 10.1371/journal.pone.0196069
• Publication Date: 2018-04-25T13:35:27Z
• Authors: Ali Amiryousefi; Jaakko Hyvönen; Péter Poczai
• Keywords: Inverted repeat; Intergenic region; Synteny

Bittersweet (Solanum dulcamara) is a native Old World member of the nightshade family. This European diploid species can be found from marshlands to high mountainous regions and it common weed that serves as an alternative host source resistance genes against plant pathogens such late blight (Phytophthora infestans). We sequenced complete chloroplast genome bittersweet, which 155,580 bp in length characterized by typical quadripartite structure composed large (85,901 bp) small (18,449 single-copy region interspersed two identical inverted repeats (25,615 bp). It consists 112 unique 81 are protein-coding, 27 tRNA four rRNA genes. All bittersweet plastid including non-functional ones even intergenic spacer transcribed primary transcripts covering 95.22% genome. These later substantially edited post-transcriptional phase activate gene functions. By comparing with all available Solanaceae sequences we content synteny highly conserved across During comparison have identified several annotation errors, corrected manual curation process then major structural changes Solanaceae. Interpreted phylogenetic context they seem provide additional support for larger clades. The sequence could help benchmark annotations used reference further studies. Such reliable important diversity calculations, map constructions assigning partitions analysis de novo plastomes