Muscle wasting in osteoarthritis model induced by anterior cruciate ligament transection

• DOI: 10.1371/journal.pone.0196682
• Publication Date: 2018-04-30T13:28:37Z
• Authors: Jordana Miranda d...; Paulo Vinicius Gi...; Vivian de Oliveir...; Eduarda Correa Fr...; Francine Hehn de ...; Rafael Mendonça d...; Ricardo Machado X...
• Keywords: Myostatin; Muscle Atrophy; Gastrocnemius muscle

This study aimed to investigate the molecular pathways involved in muscle wasting an animal model of osteoarthritis (OA) induced by anterior cruciate ligament transection (ACLT) rats. Reduction protein syntheses, increased proteolysis and impaired regeneration are important related wasting, myogenin, MyoD, myostatin MuRF-1 some their markers. Female Wistar rats were allocated into two groups: OA (submitted ACLT) SHAM surgery without ACLT). Nociception, spontaneous exploratory locomotion body weight animals evaluated weekly. Twelve weeks after disease induction, euthanized, right knee joints collected. Gastrocnemius hind paw dissected weighed. was used for evaluation atrophy expression IL-1β, TNF-α, Pax7, MuRF-1. Histopathology confirmed development group. showed a reduction about 10% area IL-1β compared Expression group, while myogenin decreased. MyoD similar both groups. Nociception significantly elevated last experimental period. Spontaneous locomotion, gastrocnemius no difference between ACLT involves within muscle, as well decreased myogenin. Therefore, may be linked regeneration.