In vitro synergistic effect of amlodipine and imipenem on the expression of the AdeABC efflux pump in multidrug-resistant Acinetobacter baumannii

• DOI: 10.1371/journal.pone.0198061
• Publication Date: 2018-06-01T17:38:52Z
• Authors: Chao Hu; Yujun Li; Ziwen Zhao; Shuquan Wei; Zhuxiang Zhao; Huiling Chen; Peilian Wu
• Keywords: Acinetobacter baumannii; Efflux; Broth microdilution

Introduction Multidrug-resistant Acinetobacter baumannii (A. baumannii) has become one of the greatest threats worldwide to therapeutic management infections. Our previous research confirmed an in vitro synergistic effect amlodipine and imipenem against A. baumannii, this study is designed understand its mechanism. Methods Sixty-four non-duplicate isolates were collected tested for antimicrobial susceptibility by disk diffusion method. PCR amplification sequencing used identify presence adeB, adeE, adeH, adeJ, abeM abeS efflux pump genes. The minimal inhibitory concentrations imipenem, imipenem+amlodipine imipenem+carbonyl cyanide m–chlorophenyl-hydrazone these also determined broth microdilution method before after siRNA silencing expression adeABC pump. Results In study, combination with showed activity sixty-four when compared alone (p<0.025). multidrug-resistant group, AML was more effective than carbonyl (p<0.001). genes detected 100% (4/64), 75% (48/64), 0% (0/64), (64/64), 96.9% (62/64) isolates, respectively. group (5.05±19.25) clearly higher non-multidrug-resistant (0.17±0.20), (p = 0.01). A gene test verified that mRNA levels decreased at 12 h increased 24 h, while reversal resistance disappeared reappeared h. Conclusions exhibits on which may be due inhibition AdeABC