Enterobacter cloacae administration induces hepatic damage and subcutaneous fat accumulation in high-fat diet fed mice

• DOI: 10.1371/journal.pone.0198262
• Publication Date: 2018-05-30T13:40:31Z
• Authors: Anniina Keskitalo; Eveliina Munukka; Raine Toivonen; Maija Hollmén; Heikki Kainulainen; Pentti Huovinen; Sirpa Jalkanen; Satu Pekkala
• Keywords: Enterobacter cloacae

Accumulating evidence indicates that gut microbiota plays a significant role in obesity, insulin resistance and associated liver disorders. Family Enterobacteriaceae especially Enterobacter cloacae strain B29 have been previously linked to obesity hepatic damage. The underlying mechanisms, however, remain unclear. Therefore, we comprehensively examined the effects of E. subsp. (ATCC® 13047™) administration on host metabolism mice fed with high-fat diet (HFD). C57BL/6N were randomly divided into HFD control, chow treatment groups. group received live bacterial cells PBS intragastrically twice week, every other week for 13 weeks. Both control groups intragastrically. After 13-week period, sacrificed gene protein expression functional analyses. Our results show increased subcutaneous fat mass relative proportion hypertrophic adipocytes. visceral had signs decreased signaling elevated lipolysis was reflected higher serum glycerol levels. In addition, -treated significantly AST AST/ALT ratio, their histology indicated fibrosis, demonstrating promotes damage mice.