The genome assembly of the fungal pathogen Pyrenochaeta lycopersici from Single-Molecule Real-Time sequencing sheds new light on its biological complexity

• DOI: 10.1371/journal.pone.0200217
• Publication Date: 2018-07-06T13:48:18Z
• Authors: Alessandra Dal Molin; Andrea Minio; Francesca Griggio; Massimo Delledonne; Alessandro Infantino; Maria Aragona
• Keywords: Inverted repeat; Illumina dye sequencing; Comparative Genomics

The first draft genome sequencing of the non-model fungal pathogen Pyrenochaeta lycopersici showed an expansion gene families associated with heterokaryon incompatibility and lacking mating-type genes, providing insights into genetic basis this "imperfect" fungus which lost ability to produce sexual stage. However, due Illumina short-read technology, was too fragmented allow a comprehensive characterization genome, especially repetitive sequence fraction. In work, another P. isolate using long-read Single Molecule Real-Time technology performed aim obtaining gapless genome. Indeed, assembly 62.7 Mb obtained, fraction sequences representing 30% total bases. content two isolates very similar, large difference in size (about 8 Mb) might be attributable high detected for new sequenced isolate. role elements, including transposable modulating virulence effectors is well established plant pathogens. Moreover, elements are fundamental importance creating re-modelling imperfect fungi. Their abundance lycopersici, together genes both isolates, suggest presence possible mechanisms alternative re-assorting mediated by recombination. A quite (~9%) has no homology known classes, strengthening hypothesis. availability allowed in-depth analysis its content, annotating functional TEs. This goal will important resource shedding light on evolution reproductive pathogenic behaviour soilborne onset speciation within species.