Impact of Leishmania donovani infection on the HLA I self peptide repertoire of human macrophages

• DOI: 10.1371/journal.pone.0200297
• Publication Date: 2018-07-12T18:01:23Z
• Authors: Lydon Wainaina Ny...; Saulius Jarmalavi...; Peter Walden
• Keywords: Antigen processing

Macrophages are specialized antigen-presenting cells that process and present self-antigens for induction of tolerance, foreign antigens to initiate T cell-mediated immunity. Despite this, Leishmania donovani (LD) able parasitize the macrophages persist. The impact this parasitizing persistence on antigen processing presentation by remains poorly defined. To gain insight into we analyzed liquid chromatography tandem mass spectrometry (LC-MS/MS) compared HLA-I self-peptidomes, proteasome compositions, HLA expression activation states non-infected LD-infected THP1-derived macrophages. We found that, though both peptidomes were dominated nonapeptides, they heterogeneous individualized, with differences in binding affinities anchor residues. Non-infected sample peptides from source proteins almost all subcellular locations involved various cellular functions, but different proportions. In infected macrophages, there was increased sampling plasma membrane extracellular proteins, those immune responses, cell communication/signal transduction metabolism/energy pathways, decreased nuclear cytoplasmic protein metabolism, RNA growth and/or maintenance. Though state unchanged, their composition altered.