Impact of manganese and heme on biofilm formation of Bacillus cereus food isolates

• DOI: 10.1371/journal.pone.0200958
• Publication Date: 2018-07-26T18:17:06Z
• Authors: Mohammad Shakhawa...; Minyeong Kwon; Deog-Hwan Oh
• Keywords: Cereus; Swarming (honey bee); Extracellular polymeric substance; Crystal violet; Swarming motility; Tryptic soy broth

The objective of this study was to determine the impact manganese (Mn2+) and heme on biofilm formation characteristics six B. cereus food isolates two reference strains (ATCC 10987 ATCC 14579). data obtained from crystal violet assay revealed that addition a combination Mn2+ BHI growth medium induced formation. However, induction strictly strain-dependent. In all strains, greater than heme. these molecules phenotypic related formation, such as cell density, sporulation swarming ability, determined in selected isolate (GIHE 72-5). Addition significantly (p < 0.05) increased number cells, which correlated with results assays well scanning electron microscopy (SEM) confocal laser (CLSM) analyses. addition, biofilms showed higher numbers spores resistance benzalkonium chloride. ability planktonic cells presence BHI. expression levels genes, are involved mobility extracellular polymeric substances (EPS) cereus, were positively These further confirming role making matrix components indicate signaling present environment might substantially trigger could pose threat industry.