The effects of insulin on the inflammatory activity of BV2 microglia

• DOI: 10.1371/journal.pone.0201878
• Publication Date: 2018-08-27T23:16:15Z
• Authors: Fiona Brabazon; Sara Bermudez; Michael Shaughness; Guzal Khayrullina; Kimberly R. Byrnes
• Keywords: Proinflammatory cytokine

Microglia are the macrophages of central nervous system (CNS), which function to monitor and maintain homeostasis. Microglial activation occurs after CNS injury, infection or disease. Prolonged microglial is detrimental as they produce nitric oxide (NO), reactive oxygen species (ROS) pro-inflammatory cytokines, resulting in neuronal cell dysfunction death. implicated neurological deficits following traumatic brain injury (TBI) Alzheimer's Intranasal insulin administration a promising treatment disease TBI. However, exact effect on microglia currently unclear. The goal this study was therefore examine activated microglia. line BV2 were exposed stimulus, lipopolysaccharide (LPS), followed by administration. Outcome measures conducted at 24 hours treatment. In vitro assays quantified NO ROS production. Western blot, immunocytochemistry phagocytosis assay further examined activity. Insulin significantly reduced NO, TNFα production increased phagocytic also iNOS expression, but had no significant any other M1 M2 macrophage polarization marker examined. These data suggest that has very specific effects reduce chemoattractant properties microglia, may be one mechanism beneficial neurodegenerative conditions.