Lipid production via simultaneous conversion of glucose and xylose by a novel yeast, Cystobasidium iriomotense

• DOI: 10.1371/journal.pone.0202164
• Publication Date: 2018-09-12T17:40:07Z
• Authors: Ayumi Tanimura; Takashi Sugita; Rikiya Endoh; Moriya Ohkuma; Shigenobu Kishino; Jun Ogawa; Jun Shima; Masako Takashima
• Keywords: Lipid Accumulation; Yeast extract

The yeast strains IPM32-16, ISM28-8sT, and IPM46-17, isolated from plant soil samples Iriomote Island, Japan, were explored in terms of lipid production during growth a mixture glucose xylose. Phylogenetically, the most closely related to Cystobasidium slooffiae, based on sequences ITS regions D1/D2 domain LSU rRNA gene. oleaginous, accumulating lipids levels > 20% dry cell weight. Moreover, kinetic analysis sugar-to-lipid conversion 1:1 glucose/xylose showed that consumed two sugars simultaneously. IPM46-17 attained highest content (33%), mostly C16 C18 fatty acids. Thus, yeasts efficiently converted lignocellulosic lipids, aiding biofuel (which benefits environment, promotes rural jobs, strengthens fuel security). constituted novel species Cystobasidium, for which we propose name iriomotense (type strain ISM28-8sT = JCM 24594T CBS 15015T).