Systematic in vitro comparison of decellularization protocols for blood vessels

• DOI: 10.1371/journal.pone.0209269
• Publication Date: 2018-12-17T18:40:16Z
• Authors: Robin Simsa; Arvind Manikantan...; Philipp Heher; Mats Hellström; Andreas Teuschl; Lachmi Jenndahl; Niklas Bergh; Per Fogelstrand
• Keywords: Decellularization; Sodium dodecyl sulfate

Decellularization of native blood vessels is a promising technology to generate 3D biological scaffolds for vascular grafting. Blood vessel decellularization has been performed in previous studies under various experimental conditions, that complicates comparison and optimization suitable protocols. The goal this work was systematically compare the recellularization efficacy 5 different protocols utilizing detergents sodium dodecyl sulfate (SDS), deoxycholate (SDC), CHAPS TritonX-100 together with DNA-removing enzymes on porcine vena cava perfusion bioreactor setup. Additionally, we tested effect DNase extracellular matrix (ECM) properties. We found all could efficiently decellularize vessels. Mechanical strength, collagen preservation ECM integrity were similar among detergents, yet TritonX required long-term application complete decellularization. However, TritonX-based showed greatest HUVECs vitro. Furthermore, developed novel protocol which improved reduced total process time stiffness compared SDS, SDC based had lower potential. In conclusion, can be achieved reagents, but treated most recellularized endothelial cells.