Understanding the impact of crosslinked PCL/PEG/GelMA electrospun nanofibers on bactericidal activity

• DOI: 10.1371/journal.pone.0209386
• Publication Date: 2018-12-20T18:38:10Z
• Authors: Mirian Michelle M...; Nicole Joy Bassous; Samson Afewerki; Samarah Vargas Harb; Paria Ghannadian; Fernanda Roberta ...; Bartolomeu Cruz V...; Carla Roberta Tim; Thomas Jay Webster; Anderson Oliveira...
• Keywords: Polycaprolactone; Biocompatibility; Electrospinning; Disinfectant; Protein Adsorption; Gelatin

Herein, we report the design of electrospun ultrathin fibers based on combination three different polymers polycaprolactone (PCL), polyethylene glycol (PEG), and gelatin methacryloyl (GelMA), their potential bactericidal activity against bacteria Staphylococcus aureus (S. aureus), Pseudomonas aeruginosa (P. aeruginosa), Methicillin-resistant (MRSA). We evaluated morphology, chemical structure wettability before after UV photocrosslinking produced scaffolds. Results showed that developed scaffolds presented hydrophilic properties PEG GelMA incorporation. Moreover, they were able to significantly reduce gram-positive, negative, MRSA mainly (PCL:PEG:GelMa-UV). Furthermore, performed a series study for gaining better mechanistic understanding through protein adsorption analysis reactive oxygen species (ROS) levels. in vivo subcutaneous implantation rats confirmed biocompatibility our designed