Molecular epidemiology and whole genome sequencing analysis of clinical Mycobacterium bovis from Ghana

• DOI: 10.1371/journal.pone.0209395
• Publication Date: 2019-03-04T18:29:44Z
• Authors: Isaac Darko Otchere; Andries J. van To...; Adwoa Asante-Poku; Leonor Sánchez-Busó; Mireia Coscollá; Stephen Osei-Wusu; Prince Asare; Samuel Yaw Aboagye; Samuel Acquah Ekuban; Abdallah Iddrisu ...; Audrey Forson; Akosua Baddoo; Clement Laryea; Julian Parkhill; Simon R. Harris; Sebastien Gagneux; Dorothy Yeboah-Manu
• Keywords: rpoB; Mycobacterium tuberculosis complex; Molecular Epidemiology

Background Bovine tuberculosis (bTB) caused by Mycobacterium bovis is a re-emerging problem in both livestock and humans. The association of some M. strains with hyper-virulence, MDR-TB disseminated disease makes it imperative to understand the biology pathogen. Methods (15) among 1755 complex (MTBC) isolated between 2012 2014 were characterized analyzed for associated patient demography other risk factors. Five isolates whole-genome sequenced comparatively against global collection published genomes. Results was from 3/560(0.5%) females 12/1195(1.0%) males pulmonary TB. average age infected cases 46.8 years (7-72years). TB patients Northern region Ghana (1.9%;4/212) had higher rate infection (OR = 2.7,p 0.0968) compared those Greater Accra (0.7%;11/1543). Among available HIV status, odds isolating (2/119) 3.3 relative non-HIV (4/774). Direct contact or their unpasteurized products significantly bTB (p<0.0001, OR 124.4,95% CI 30.1–508.3). Two (13.3%) INH resistant due S315T mutation katG whereas one (6.7%) RIF Q432P I1491S mutations rpoB. resolved as mono-phyletic branch mostly Africa irrespective host closest root phylogeny. bovis-specific amino acid detected MTBC core genes such mce1A, mmpL1, pks6, phoT, pstB, glgP Rv2955c. Additional P6T chaA, G187E mgtC, T35A Rv1979c, S387A narK1, L400F fas A563T eccA1 restricted 5 clinical Ghana. Conclusion Our data indicate potential zoonotic transmission hence calls intensified public education on bTB, especially groups.