The successful containment of a hospital outbreak caused by NDM-1-producing Klebsiella pneumoniae ST307 using active surveillance

• DOI: 10.1371/journal.pone.0209609
• Publication Date: 2019-02-13T19:06:20Z
• Authors: Paola Bocanegra-I...; Elvira Garza-Gonz...; Magaly Padilla-Or...; Soraya Mendoza-Ol...; Eduardo Pérez-Alba; Samantha Flores-T...; Ulises Garza-Ramos; Jesus Silva-Sánchez; Adrián Camacho-Ortiz
• Keywords: Klebsiella infections

The worldwide dissemination of high-risk carbapenemase-producing Klebsiella pneumoniae clones has become a major threat to healthcare facilities. This study describes the successful containment hospital outbreak caused by NDM-1-producing K. Sequence Type (ST) 307 using active surveillance. began when patient was transferred from local hospital. After 48 hours in our hospital, tracheal aspirate positive for meropenem resistant and pneumoniae. All patients medical intensive care unit (ICU) neurology wards were subject contact precautions. surfaces devices, workers, these screened cultures. Fecal swabs placed into broth PCR blaKPC, blaOXA-48, blaIMP, blaVIM, blaNDM, which performed directly after 12 hours. PCRs also on DNA extracted species subcultured broths. Five nine days later, two more patients' rectal tested positive. Molecular assays identified blaNDM-1 onto 130-kb conjugative plasmid (IncY, IncFIIs, IncFIIY), ST307. three discharged, monitoring continued, weeks with negative results, swabbing ended. In conclusion, it possible contain ST307 through epidemiological microbiological With methodology used, detection NDM-type genes fecal samples obtained approximately 15 obtaining sample.