Heterosis and combining ability in cytoplasmic male sterile and doubled haploid based Brassica oleracea progenies and prediction of heterosis using microsatellites

• DOI: 10.1371/journal.pone.0210772
• Publication Date: 2019-08-19T17:27:23Z
• Authors: Saurabh Singh; S. S. Dey; Reeta Bhatia; Raj Kumar; Kanika Sharma; T. K. Behera
• Keywords: Doubled haploidy; Genetic distance

In Brassica oleracea, heterosis is the most efficient tool providing impetus to hybrid vegetable industry. this context, we presented first report on identifying superior heterotic crosses for yield and commercial traits in cauliflower involving cytoplasmic male sterile (CMS) doubled haploid (DH) lines as parents. We studied suitability of genomic-SSRs EST-SSRs based genetic distance (GD) agronomic trait phenotypic (PD) predicting F1 hybrids using CMS DH 120 derived from 20Ogura 6 testers were evaluated 16 along with 26 parental 4 standard checks. The structure analysis grouped into distinct clusters. Ogu118-6A, Ogu33A, Ogu34-1A good general combiner developing early maturity hybrids. SCA effects significantly associated suggesting non-additive gene response Less than unity value σ2A/D coupled σ2gca/σ2sca indicated predominance action expression traits. correlation suggested that microsatellites estimates can be helpful prediction some extent.