Modulation of microbial communities and mucosal gene expression in chicken intestines after galactooligosaccharides delivery In Ovo

• DOI: 10.1371/journal.pone.0212318
• Publication Date: 2019-02-27T19:24:12Z
• Authors: Anna Slawinska; Aleksandra Dunisl...; Arkadiusz Plowiec; Malgorzata Radomska; Jagoda Lachmanska; Maria Siwek; Siria Tavaniello; Giuseppe Maiorano
• Keywords: In ovo; Caecum; Jejunum; Cecum; Bifidobacterium animalis; Mucin 2; Intestinal mucosa; Prebiotic

Intestinal mucosa is the interface between microbial content of gut and host's milieu. The goal this study was to modulate chicken intestinal microflora by in ovo stimulation with galactooligosaccharides (GOS) prebiotic demonstrate molecular responses host. animal trial performed on meat-type chickens (Ross 308). GOS delivered injection into air cell day 12 egg incubation. Analysis communities mucosal gene expression at slaughter (day 42 post-hatching). Chyme (for DNA isolation) RNA from four distinct segments (duodenum, jejunum, ileum, caecum) sampled. relative abundance Bifidobacterium spp. Lactobacillus isolated chyme samples determined using qPCR. On host side, mRNA 13 genes grouped two panels analysed RT-qPCR. Panel (1) included related innate immune (IL-1β, IL-10 IL-12p40, AvBD1 CATHL2). (2) contained involved barrier function (MUC6, CLDN1 TJAP1) nutrients sensing (FFAR2 FFAR4, GLUT1, GLUT2 GLUT5). increased caecum (from 1.3% 3.9%). Distinct effects were manifested jejunum caecum. Cytokine IL-12p40) up-regulated GOS-treated group. Host defence peptides (AvBD1 CATHL2) Free fatty acid receptors FFAR4) all three compartments intestine (except duodenum). Glucose transporters down-regulated duodenum (GLUT2 GLUT5) but hindgut (GLUT1 GLUT2). In conclusion, had a bifidogenic effect adult chickens. It also modulated responses, function, nutrient sensing.