Hydrogen gas promotes the adventitious rooting in cucumber under cadmium stress

• DOI: 10.1371/journal.pone.0212639
• Publication Date: 2019-02-20T19:39:59Z
• Authors: Bo Wang; Biting Bian; Chunlei Wang; Changxia Li; Hua Fang; Jing Zhang; Dengjing Huang; Jianqiang Huo; Weibiao Liao
• Keywords: 0303 health sciences; Science; Q; R; Plant Roots; 6. Clean water; Oxidative Stress; 03 medical and health sciences; Osmoregulation; Stress, Physiological; Medicine; Cucumis sativus; Research Article; Cadmium; Hydrogen

Hydrogen gas (H2) plays an important role in plant development and stress responses. Here, cucumber (Cucumis sativus L.) explants were used to investigate the roles of H2 in adventitious root development under cadmium (Cd) stress and its physiological mechanism. The results showed that hydrogen-rich water (HRW) promoted adventitious rooting under Cd stress and 50% HRW obtained the maximal biological response. Compared with Cd treatment, HRW + Cd treatment significantly reduced the content of malondialdehyde (MDA), hydrogen peroxide (H2O2), superoxide radical (O2-), thiobarbituric acid reactive substances (TBARS), ascorbic acid (AsA) and reduced glutathione (GSH), as well as relative electrical conductivity (REC), lipoxygenase (LOX) activity, AsA/docosahexaenoic acid (DHA) ratio, and GSH/oxidized glutathione (GSSG) ratio, while increasing DHA and GSSG content. HRW + Cd treatment also significantly increased in the activity and related gene expression of ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR) and glutathione reductase (GR). Additionally, HRW + Cd treatment increased the contents of osmotic adjustment substances, as well as the activities of peroxidase (POD) and polyphenol oxidase (PPO), while significantly decreasing indoleacetic acid oxidase (IAAO) activity. In summary, H2 could induce adventitious rooting under Cd stress by decreasing the oxidative damage, increasing osmotic adjustment substance content and regulating rooting-related enzyme activity.