Methods of olfactory ensheathing cell harvesting from the olfactory mucosa in dogs

• DOI: 10.1371/journal.pone.0213252
• Publication Date: 2019-03-06T20:21:48Z
• Authors: Daisuke Ito; Darren Carwardine; Jon Prager; Liang Fong Wong; Masato Kitagawa; Nick Jeffery; Nicolas Granger
• Keywords: Olfactory ensheathing glia; Olfactory mucosa

Olfactory ensheathing cells are thought to support regeneration and remyelination of damaged axons when transplanted into spinal cord injuries. Following transplantation, improved locomotion has been detected in many laboratory models dogs with naturally-occurring injury; safety trials humans have also completed. For widespread clinical implementation, it will be necessary derive large numbers these from an accessible and, preferably, autologous, source making olfactory mucosa a good candidate. Here, we compared the yield using 3 different techniques: rhinotomy, frontal sinus keyhole approach rhinoscopy. From canine cases injury, 27 biopsies were obtained by 7 1 Biopsy via rhinoscopy was tested 13 cadavers living normal dogs. After 21 days cell culture, proportions populations p75-positive (presumed ensheathing) similar (~4.5 x 106 cells; ~70% total population), but fewer rhinotomy. Cerebrospinal fluid rhinorrhea observed one dog emphysema following Blepharitis occurred after approach. All three biopsy methods appear safe for harvesting suitable number transplantation within each technique specific advantages drawbacks.