Genome sequencing and transcript analysis of Hemileia vastatrix reveal expression dynamics of candidate effectors dependent on host compatibility

• DOI: 10.1371/journal.pone.0215598
• Publication Date: 2019-04-18T17:43:56Z
• Authors: Brenda Neves Porto; Eveline Teixeira ...; Sandra Marisa Mat...; Pedro Marcus Pere...; Laércio Zambolim; Eunize Maciel Zam...; Nicole Donofrio; Shawn W. Polson; Thiago Andrade Maia; Chuming Chen; Modupe Adetunji; Brewster Kingham; Ronaldo José Duri...; Mário Lúcio Vilel...
• Keywords: Haustorium; Proteome; Gene prediction

Coffee leaf rust caused by the fungus Hemileia vastatrix is one of most important diseases coffee plantations worldwide. Current knowledge H. genome limited and only a small fraction total fungal secretome has been identified. In order to obtain more comprehensive understanding its secretome, we aimed sequence assemble entire using two next-generation sequencing platforms hybrid assembly strategy. This resulted in 547 Mb race XXXIII (Hv33), with 13,364 predicted genes that encode 13,034 putative proteins transcriptomic support. Based on this proteome, 615 contain secretion peptides, lack transmembrane domains. From 111 were identified as candidate effectors (EHv33) unique vastatrix, subset consisting 17 EHv33 was selected for temporal gene expression analysis during infection. Five significantly induced early an incompatible interaction, indicating their potential role pre-haustorial possibly recognized resistant genotype. Another nine after haustorium formation compatible interaction. Overall, suggest able selectively mount survival strategy depend host genotype involved infection process.