Alternative polyadenylation of single cells delineates cell types and serves as a prognostic marker in early stage breast cancer

• DOI: 10.1371/journal.pone.0217196
• Publication Date: 2019-05-17T17:28:27Z
• Authors: Nayoung Kim; Woosung Chung; Hye Hyeon Eum; Hae-Ock Lee; Woong-Yang Park
• Keywords: Cell type

Alternative polyadenylation (APA) in 3' untranslated regions (3' UTR) plays an important role regulating transcript abundance, localization, and interaction with microRNAs. Length-variation of 3'UTRs by APA contributes to efficient proliferation cancer cells. In this study, we investigated single cells tumor microenvironment understand the physiological implication different cell types. We analyzed patterns expression level genes from 515 single-cell RNA sequencing (scRNA-seq) dataset 11 breast patients. Although overall 3'UTR length individual was distributed equally non-tumor cells, found a differential pattern gene sets between addition, across types using scRNA-seq data 3 glioblastoma patients 1 renal carcinoma detail, 1,176 53 showed distinct shortening over-expression as signatures for five including B lymphocytes, T myeloid stromal Functional categories cellular demonstrated concordant regulation specific The significantly correlated clinical outcome earlier stage identified type-specific which allows identification based on variation combination expression. Specifically, immune-specific signature could be utilized prognostic marker early cancer.