Providence virus: An animal virus that replicates in plants or a plant virus that infects and replicates in animal cells?

• DOI: 10.1371/journal.pone.0217494
• Publication Date: 2019-06-04T17:27:01Z
• Authors: Meesbah Jiwaji; Gwynneth Felicity...; Mart-Mari de Bruyn; Janet Awino Awando; Holisha Moodley; Dylan Waterworth; Rachel Anne Jarvie; Rosemary Ann Dorr...
• Keywords: 0301 basic medicine; Science; Vigna; Q; R; 3. Good health; Lepidoptera; 03 medical and health sciences; RNA Virus Infections; Plant Cells; MCF-7 Cells; Medicine; Animals; Humans; RNA Viruses; Research Article; HeLa Cells

Emerging viral diseases, most of which are zoonotic, pose a significant threat to global health. There is a critical need to identify potential new viral pathogens and the challenge is to identify the reservoirs from which these viruses might emerge. Deep sequencing of invertebrate transcriptomes has revealed a plethora of viruses, many of which represent novel lineages representing both plant and animal viruses and little is known about the potential threat that these viruses pose.Providence virus, an insect virus, was used to establish a productive infection in Vigna unguiculata (cowpea) plants. Providence virus particles purified from these cowpea plants were used to infect two mammalian cell lines.Here, we present evidence that Providence virus, a non-enveloped insect RNA virus, isolated from a lepidopteran midgut cell line can establish a productive infection in plants as well as in animal cells. The observation that Providence virus can readily infect both plants and mammalian cell culture lines demonstrates the ability of an insect RNA virus to establish productive infections across two kingdoms, in plants and invertebrate and vertebrate animal cell lines.The study highlights the potential of phytophagous insects as reservoirs for viral re-assortment and that plants should be considered as reservoirs for emerging viruses that may be potentially pathogenic to humans.