Identification of L-Valine-initiated-germination-active genes in Bacillus subtilis using Tn-seq

• DOI: 10.1371/journal.pone.0218220
• Publication Date: 2019-06-14T17:38:49Z
• Authors: Cameron V. Sayer; Bidisha Barat; David L. Popham
• Keywords: Spore germination

Bacterial endospores can survive harsh environmental conditions and long-term dormancy in the absence of nutrients, but rapidly germinate under favorable conditions. In present study, we employed transposon sequencing (Tn-seq) to identify genes with previously uncharacterized roles spore germination. Identified that encoded inner membrane proteins were chosen for study defined mutants, which exhibited delayed germination several assays response varying germinants. Significantly slowed release DPA indicated mutants affected Stage I Several phenotypic traits consistent failure a GerA germinant receptor-mediated response, while others appeared have more general loss varied Use gerA-lacZ transcriptional fusion quantitative western blotting GerAC allowed be classified based upon normal or decreased gerA transcription reduced accumulation. Fourteen identified newly described within Bacillus A complete understanding this process contribute development better decontamination procedures.