MicroRNA expression profile in retina and choroid in oxygen-induced retinopathy model

• DOI: 10.1371/journal.pone.0218282
• Publication Date: 2019-06-12T17:37:16Z
• Authors: Michel Desjarlais; Jose Carlos Rivera; Isabelle Lahaie; Gaël Cagnone; Maëlle Wirt; Samy Omri; Sylvain Chemtob
• Keywords: Choroid

Ischemic retinopathies (IRs) are leading causes of visual impairment. They characterized by an initial phase microvascular degeneration and a second aberrant pre-retinal neovascularization (NV). microRNAs (miRNAs) regulate gene expression, number play role in normal pathological NV. But, post-transcriptional modulation miRNAs the eye during development IRs has not been systematically evaluated.Using Next Generation Sequencing (NGS) we profiled miRNA expression retina choroid vasodegenerative NV phases oxygen-induced retinopathy (OIR).Approximately 20% total exhibited altered (up- or down-regulation); 6% were found highly expressed rats subjected to OIR. During OIR-induced vessel phase, miR-199a-3p, -199a-5p, -1b, -126a-3p displayed robust decreased (> 85%) retina. While choroid, miR-152-3p, -142-3p, -148a-3p, -532-3p upregulated (>200%) miR-96-5p, -124-3p, -9a-3p, -190b-5p, -181a-1-3p, -9a-5p, -183-5p downregulated (>70%) compared controls. peak NV, miR-30a-5p, -30e-5p 190b-5p markedly reduced (>70%), miR-30e-3p, miR-335, -30b-5p strongly augmented (by up 300%) Whereas miR-let-7f-5p, miR-126a-5p miR-101a-3p (>81%), miR-125a-5p, let-7e-5p let-7g-5p (>570%) Changes observed using NGS validated qRT-PCR for 24 most modulated miRNAs. In silico approach predict target genes (using algorithms miRSystem database) identified potential new with pro-inflammatory, apoptotic angiogenic properties.The present study is first comprehensive description retinal/choroidal profiling OIR technology). Our results provide valuable framework characterization possible therapeutic specific involved ocular IR-triggered inflammation, angiogenesis degeneration.