Utility of chemokines CCL2, CXCL8, 10 and 13 and interleukin 6 in the pediatric cohort for the recognition of neuroinflammation and in the context of traditional cerebrospinal fluid neuroinflammatory biomarkers

• DOI: 10.1371/journal.pone.0219987
• Publication Date: 2019-07-29T13:34:24Z
• Authors: Zuzana Liba; Hana Nohejlova; Vaclav Capek; Pavel Krsek; Anna Sediva; Jana Kayserova
• Keywords: Lymphocytic pleocytosis; Pleocytosis

Background The recognition of active inflammation in the central nervous system (CNS) absence infectious agents is challenging. present study aimed to determine diagnostic relevance five selected chemo/cytokines CNS and context traditional cerebrospinal fluid (CSF) biomarkers (white blood cell [WBC] counts, oligoclonal bands, protein levels, CSF/serum albumin ratios) clinical diagnoses. Methods C-C C-X-C motif ligands (CCL2, CXCL8, 10 13) interleukin (IL) 6 levels CSF serum from 37 control 87 symptomatic children with ten different (mostly noninfectious) inflammatory disorders (16 which had follow-up samples after recovery) were determined using Luminex multiple bead technology software. Nonparametric tests used; p < 0.05 was considered statistically significant. Receiver operating characteristic curves constructed analyze controls 1) all or 2) without pleocytosis. Results Compared samples, investigated increased only IL-6 remained elevated recovery (p ≤ 0.001). CXCL-13 (> 10.9 pg/mL) best individual discriminatory criterion differentiate neuroinflammation (specificity/sensitivity: 97/72% 97/61% for pleocytosis), followed by WBC counts 97/62%). utility remaining chemo/cytokine descending order sensitivities corresponding thresholds that ensured 97% specificity pleocytosis (pg/mL; sensitivity %): (3.8; 34), CXCL8 (32; 26), CXCL10 (317; 24) CCL2 (387; 10). Different diagnosis-related patterns observed. Conclusions level CXCL13 marker greatest predictive general among individually biomarkers. potential differential diagnosis neuroinflammatory diseases identified.