TSG-6 in extracellular vesicles from canine mesenchymal stem/stromal is a major factor in relieving DSS-induced colitis

• DOI: 10.1371/journal.pone.0220756
• Publication Date: 2020-02-10T13:24:01Z
• Authors: Ju-Hyun An; Qiang Li; Min-Ok Ryu; A-Ryung Nam; Dong-Ha Bhang; Yun-Chan Jung; Woo-Jin Song; Hwa-Young Youn
• Keywords: Macrophage polarization; Proinflammatory cytokine

Adipose tissue derived mesenchymal stem/stromal cell (ASC)-derived extracellular vesicles (EV) have been reported to be beneficial against dextran sulfate sodium (DSS)-induced colitis in mice. However, the underlying mechanisms not fully elucidated. We hypothesize that tumor necrosis factor-α-stimulated gene/protein 6 (TSG-6) EVs is a key factor influencing alleviation of symptoms. DSS-induced mice (C57BL/6, male, Naïve = 6, Sham 8, PBS 8 EV CTL-EV TSG-6 depleted 8) were intraperitoneally administered (100 ug/mice) on day 1, 3, and 5; colon tissues collected 10 for histopathological, RT-qPCR, western blot immunofluorescence analyses. In injected with EV, inflammation was alleviated. Indeed, regulated levels pro- anti-inflammatory cytokines, such as TNF-α, IL-1β, IFN-γ, IL-6, IL-10 inflamed colons. when degree inflammatory relief reduced. Furthermore, plays role increasing regulatory T cells (Tregs) polarizing macrophage from M1 M2 colon. conclusion, this study shows major colitis, by number Tregs polarization