Graft glycocalyx degradation in human liver transplantation

• DOI: 10.1371/journal.pone.0221010
• Publication Date: 2019-08-15T17:37:28Z
• Authors: Arie Passov; Alexey Schramko; Heikki Mäkisalo; Arno Nordin; Sture Andersson; Eero Pesonen; Minna Ilmakunnas
• Keywords: Glycocalyx; Syndecan 1

Objective Ischaemia/reperfusion-injury degrades endothelial glycocalyx. Graft glycocalyx degradation was studied in human liver transplantation. Methods To assess changes within the graft, blood drawn from portal and hepatic veins addition to systemic samples 10 patients. Plasma syndecan-1, heparan sulfate chondroitin sulfate, were measured with enzyme-linked immunosorbent assay. Results During reperfusion, syndecan-1 levels higher graft caval effluent [3118 (934–6141) ng/ml, P = 0.005] than venous [101 (75–121) ng/ml], indicating release graft. Concomitantly, lower [96 (32–129) 0.037] [112 (98–128) uptake Chondroitin equal blood. After reperfusion arterial increased 17-fold (P < 0.001) decreased a third towards end of surgery. Conclusion Syndecan-1 washout indicates extensive during reperfusion. Surprisingly, taken up by Corroborating previous experimental reports, this suggests that endogenous might be utilized repair damaged