Host plants influence the composition of the gut bacteria in Henosepilachna vigintioctopunctata

• DOI: 10.1371/journal.pone.0224213
• Publication Date: 2019-10-18T17:37:52Z
• Authors: Jing Lü; Wei Guo; Shimin Chen; Mujuan Guo; Baoli Qiu; Chunxiao Yang; Tengxiang Lian; Huipeng Pan
• Keywords: Verrucomicrobia

The gut bacteria of insects positively influence the physiology their host, however, dynamics this complicated ecosystem are not fully clear. To improve our understanding, we characterized prokaryotic Henosepilachna vigintioctopunctata that fed on two host plants, Solanum melongena (referred to as QZ hereafter) and nigrum LK hereafter), by sequencing V3-V4 hypervariable region 16S rRNA gene using Illumina MiSeq system. results revealed bacterial composition varied between specimens different plants. unweighted pair group method with arithmetic mean analyses principal coordinate analysis showed communities groups were distinct. Four phyla (Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria) present in all H. samples. It is noteworthy phylum Cyanobacteria only found group, a low relative abundance. Proteobacteria Enterobacteriaceae predominant family, respectively, both groups. Linear discriminant effect size (LEfSe) enriched Bacilli class Lactococcus genus; while Alphaproteobacteria Ochrobactrum genus. PICRUSt genes predicted be involved xenobiotic biodegradation metabolism, metabolism other amino acids, signaling molecules, interaction significantly higher group. Genes cofactors vitamins Furthermore, complexity network structure modularity than This first study characterize vigintioctopunctat, demonstrate plants tested had considerable impact dominated relatively few taxa.