Genome-wide histone modification profiling of inner cell mass and trophectoderm of bovine blastocysts by RAT-ChIP

• DOI: 10.1371/journal.pone.0225801
• Publication Date: 2019-11-25T13:30:57Z
• Authors: Tõnis Org; Kati Hensen; Rita Kreevan; Elina Mark; Olav Sarv; Reidar Andreson; Ülle Jaakma; Andres Salumets; Ants Kurg
• Keywords: Chromatin immunoprecipitation; H3K4me3; Inner cell mass; Histone code

Chromatin immunoprecipitation coupled with next-generation sequencing (ChIP-seq) has revolutionized our understanding of chromatin-related biological processes. The method, however, requires thousands cells and therefore limited applications in situations where cell numbers are limited. Here we describe a novel method called Restriction Assisted Tagmentation Immunoprecipitation (RAT-ChIP) that enables global histone modification profiling from as few 100 cells. is simple, cost-effective takes single day to complete. We demonstrate the sensitivity by deriving first genome-wide maps H3K4me3 H3K27me3 modifications inner mass trophectoderm bovine blastocyst stage embryos.