Pharmacokinetics of a single inhalation of hydrogen gas in pigs

• DOI: 10.1371/journal.pone.0234626
• Publication Date: 2020-06-19T20:05:47Z
• Authors: Motoaki Sano; Genki Ichihara; Yoshinori Katsumata; Takahiro Hiraide; Akeo Hirai; Mizuki Momoi; Tomoyoshi Tamura; Shigeo Ohata; Eiji Kobayashi
• Keywords: Blood sampling; Venous blood; Arterial blood

The benefits of inhaling hydrogen gas (H2) have been widely reported but its pharmacokinetics not yet sufficiently analyzed. We developed a new experimental system in pigs to closely evaluate the process by which H2 is absorbed lungs, enters bloodstream, and distributed, metabolized, excreted. inserted secured catheters into carotid artery (CA), portal vein (PV), supra-hepatic inferior vena cava (IVC) allow repeated blood sampling performed bilateral thoracotomy collapse lungs. Then, using hydrogen-absorbing alloy canister, we filled lungs maximum inspiratory level with 100% H2. pig was maintained for 30 seconds without resuming breathing, as if they were holding their breath. collected from three intravascular after 0, 3, 10, 30, 60 minutes measured concentration chromatography. CA peaked immediately breath holding; 3 min later, it dropped 1/40 peak value. Peak concentrations PV IVC 40% 14% that CA, respectively. However, decay (half-life: 310 s 350 s, respectively) slower than 92 s). At 10 min, significantly higher venous arterial blood. detected at 6.9–53 nL/mL steady state, SVC 14–29 state. In contrast, decreased state levels. This first report showing inhaled transported whole body advection diffusion metabolized dynamically.