Molecular typing and characterization of a novel genotype of EV-B93 isolated from Tibet, China

• DOI: 10.1371/journal.pone.0237652
• Publication Date: 2020-08-25T17:30:40Z
• Authors: Man Zhang; Yong Zhang; Mei Hong; Jinbo Xiao; Zhenzhi Han; Yang Song; Shuangli Zhu; Dongmei Yan; Qian Yang; Wenbo Xu; Zhijun Liu
• Keywords: Molecular Epidemiology; Strain (injury); Multilocus sequence typing

EV-B93 is a novel serotype within the Enterovirus B species and uncommon worldwide. Currently, only one full-length genomic sequence (the prototype strain) has been deposited in GenBank database. In this study, three were identified, including from an acute flaccid paralysis (AFP) patient (named 99052/XZ/CHN/1999, hereafter XZ99052) two healthy children 99096/XZ/CHN/1999 99167/XZ/CHN/1999, XZ99096 XZ99167, respectively) Tibet 1999 during polio eradication program. The identity between nucleotide amino acid sequences of strain 83.2%-83.4% 96.8%-96.9%, respectively. was found to have greater P3 region another enterovirus EV-B107 as per phylogenetic tree analysis, which revealed that recombination occurred. Seroepidemiology data showed not produced epidemic there may be susceptible individuals. strains are temperature-resistant with prognosticative virulence, suggesting possibility potential large-scale outbreak EV-B93. analyzed enrich our knowledge about provide valuable information on global molecular epidemiology. What more, they permit appraisal serotype's public health impact aid understanding role events evolution enteroviruses.