A comparison of non-magnetic and magnetic beads for measuring IgG antibodies against Plasmodium vivax antigens in a multiplexed bead-based assay using Luminex technology (Bio-Plex 200 or MAGPIX)

• DOI: 10.1371/journal.pone.0238010
• Publication Date: 2020-12-04T19:32:07Z
• Authors: Ramin Mazhari; Jessica Brewster; Rich Fong; Caitlin Bourke; Zoe S. J. Liu; Eizo Takashima; Takafumi Tsuboi; Wai-Hong Tham; Matthias Harbers; Chetan Chitnis; Julie Healer; Maria Ome-Kaius; Jetsumon Sattabon...; James Kazura; Leanne J. Robinson; Christopher King; Ivo Mueller; Rhea J. Longley
• Keywords: Bead; Magnetic bead; Magnetic separation; Immunomagnetic separation

Multiplexed bead-based assays that use Luminex ® xMAP technology have become popular for measuring antibodies against proteins of interest in many fields, including malaria and more recently SARS-CoV-2/COVID-19. There are currently two formats widely used: non-magnetic beads or magnetic beads. Data lacking regarding the comparability results obtained using these types beads, run on different instruments. Whilst can only be flow-based instruments (such as 100/200™ Bio-Plex 200), both newer MAGPIX In this study we utilized a panel purified recombinant Plasmodium vivax samples from malaria-endemic areas to measure P . -specific IgG responses combinations We directly compared: i) versus 200, ii) 200 iii) also performed an external comparison our optimized assay. observed antibody responses, measured proteins, were moderately-strongly correlated all three comparisons (pearson r>0.5 18/19 proteins), however higher amounts protein required coupling Our indicated generated laboratories same coupled highly comparable r>0.7), particularly if reference standard curve is used.