Evaluation of the GenoType NTM-DR assay performance for the identification and molecular detection of antibiotic resistance in Mycobacterium abscessus complex

• DOI: 10.1371/journal.pone.0239146
• Publication Date: 2020-09-25T17:32:55Z
• Authors: Nicolas Bouzinbi; Olivier Marcy; Thibault Bertolotti; Raphael Chiron; Pascale Bemer; Martine Pestel-Caron; Olivia Peuchant; Hélène Guet-Revillet; Marie-Sarah Fangous; Geneviève Héry-Ar...; Abdoul-Salam Oued...; Anne-Laure Bañuls; Sylvain Godreuil
• Keywords: DNA, Bacterial; 0301 basic medicine; 570; Genotyping Techniques; [SDV]Life Sciences [q-bio]; Science; DNA Mutational Analysis; 610; Mycobacterium Infections, Nontuberculous; Microbial Sensitivity Tests; Polymerase Chain Reaction; 630; 03 medical and health sciences; Clarithromycin; Drug Resistance, Bacterial; Humans; Amikacin; 0303 health sciences; Mycobacterium abscessus; Q; R; Anti-Bacterial Agents; 3. Good health; [SDV] Life Sciences [q-bio]; Genes, Bacterial; Mutation; Medicine; Reagent Kits, Diagnostic; Research Article; Multilocus Sequence Typing

The first objective of this study was to determine the GenoType NTM-DR assay performance for subspecies identification in Mycobacterium abscessus complex isolates. The second objective was to evaluate the GenoType NTM-DR assay ability to detect clarithromycin and amikacin resistance in M. abscessus complex isolates compared with drug susceptibility testing (DST) and PCR sequencing of the erm(41), rrl and rrs genes. The concordance between the GenoType NTM-DR and MLST results concerning subspecies identification was 100%. The wild type and mutated alleles of the rrl and rrs genes were detected by the GenoType NTM-DR assay and PCR sequencing with 100% (115/115) agreement. Similarly, 100% concordance between GenoType NTM-DR and DST was observed for clarithromycin and amikacin testing. Sensitivity for the detection of clarithromycin and amikacin resistance was 100%. The GenoType NTM-DR assay provides a robust and complementary tool to the gold standard methods (MLST and broth microdilution) for subspecies identification and drug resistance detection.