Identification and characterization of Miscanthus yellow fleck virus, a new polerovirus infecting Miscanthus sinensis

• DOI: 10.1371/journal.pone.0239199
• Publication Date: 2020-09-17T17:56:37Z
• Authors: Stephen Bolus; Martha Malapi-Wight; Samuel C. Grinstead; Irazema Fuentes-B...; Leticia Hendrickson; Rosemarie W. Hammond; Dimitre Mollov
• Keywords: Miscanthus sinensis; ORFS

Miscanthus sinensis is a grass used for sugarcane breeding and bioenergy production. Using high throughput sequencing technologies, we identified new viral genome in infected M. leaf tissue displaying yellow fleck symptoms. This virus most related to members of the genus Polerovirus family Luteoviridae. The canonical ORFs were computationally identified, P3 coat protein was expressed, virus-like particles purified found conform icosahedral shapes, characteristic We propose name this virus.