Role of a complex of two proteins in alleviating sodium ion stress in an economic crop

• DOI: 10.1371/journal.pone.0242221
• Publication Date: 2020-11-20T18:54:16Z
• Authors: Jie Yang; Mingyu Liu
• Keywords: Ions; Nicotiana; 0301 basic medicine; 2. Zero hunger; Serotonin; Science; Q; R; Sodium Chloride; 15. Life on land; Plants, Genetically Modified; Plant Roots; Plant Leaves; 03 medical and health sciences; Cajanus; Gene Expression Regulation, Plant; Stress, Physiological; Medicine; Phylogeny; Research Article; Plant Proteins

An economically valuable woody plant species tree bean (Cajanus cajan (L.) Millsp.) is predominantly cultivated in tropical and subtropical areas and is regarded as an important food legume (or pulse) crop that is facing serious sodium ion stress. NAM (N-acetyl-5-methoxytryptamine) has been implicated in abiotic and biotic stress tolerance in plants. However, the role of NAM in sodium ion stress tolerance has not been determined. In this study, the effect of NAM was investigated in the economically valuable woody plant species, challenged with stress at 40 mM sodium ion for 3 days. NAM-treated plants (200 μM) had significantly higher fresh weight, average root length, significantly reduced cell size, increased cell number, and increased cytoskeleton filaments in single cells. The expression pattern of one of 10 Tree bean Dynamic Balance Movement Related Protein (TbDMP), TbDMP was consistent with the sodium ion-stress alleviation by NAM. Using TbDMP as bait, Dynamic Balance Movement Related Kinase Protein (TbDBK) was determined to interact with TbDMP by screening the tree bean root cDNA library in yeast. Biochemical experiments showed that NAM enhanced the interaction between the two proteins which promoted resist sodium ion stress resistance. This study provides evidence of a pathway through which the skeleton participates in NAM signaling.