Cytogenetic characterization and mapping of the repetitive DNAs in Cycloramphus bolitoglossus (Werner, 1897): More clues for the chromosome evolution in the genus Cycloramphus (Anura, Cycloramphidae)

• DOI: 10.1371/journal.pone.0245128
• Publication Date: 2021-01-13T19:03:11Z
• Authors: Gislayne de Paula...; Kaleb Pretto Gatto; Camilla Borges Ga...; Peterson T. Leivas; Michelle M. Struett; Maurício Moura; Daniel Pacheco Br...
• Keywords: Satellite DNA; Concerted evolution

Cycloramphus bolitoglossus (Werner, 1897) is a rare species with low population density in the Serra do Mar region of Paraná and Santa Catarina, southern Brazil. Currently, it has been assigned to Near Threatened (NT) category Brazilian List Endangered Animal Species. Here, we described karyotype this for first time investigated patterns some repetitive DNA classes chromosomes using molecular cytogenetic approaches. We isolated, sequenced mapped 5S rDNA satellite PcP190 C . , as well telomeric sequences seven microsatellites motifies [(GA) 15 (CA) (GACA) 4 (GATA) 8 (CAG) 10 (CGC) (GAA)] 2n = 26 fundamental number (FN) equal 52, highly conserved compared other genus members. Comparative under phylogenetic context allowed evolutionary interpretations morphological changes homologs pairs 1, 3, 6 along history Two subtypes type II were isolated genome, several comparative analysis suggests mixed effects concerted birth-and-death evolution acting DNA. The subtype “a” “b” was on chromosome 1. However, their different position 1 provide an excellent marker future studies. DNA, already reported families Hylidae, Hylodidae, Leptodactylidae, Odontophrynidae, scattered throughout even non-heterochromatic regions showed hybridization signals probe. Molecular that exhibit high-level homogenization sequence genome satDNA from represents novel group, anurans, based its hypervariable region. Overall, present data pseudogenization evidence corroborated hypothesis emergence clusters. These two arguments reinforced importance model explain found anuran genomes.