Fine mapping epitope on Glycoprotein-Gn from Severe Fever with Thrombocytopenia Syndrome Virus

• DOI: 10.1371/journal.pone.0248005
• Publication Date: 2021-03-02T18:49:13Z
• Authors: Abulimiti Moming; Shen Shi; Shu Shen; Jie Qiao; Xihong Yue; Bo Wang; Juntao Ding; Zhihong Hu; Fei Deng; Yujiang Zhang; Surong Sun
• Keywords: Epitope mapping; Linear epitope

Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) was recently identified as a tick-borne pathogen that threat to human health. Since 2010, many countries including China, South Korea, and Japan have reported Human SFTS caused by SFTSV infection. The glycoprotein encoded the M gene is major antigenic component on viral surface, responsible for entry, which makes it an important antigen clinical diagnostic target. present study aimed map linear B cell epitopes (BCEs) N-terminal (Gn) from strain WCH/97/HN/China/2011 using modified biosynthetic peptide method. Five fine (E1, 196 FSQSEFPD 203 ; E2, 232 GHSHKII 238 E3, 256 VCYKEGTGPC 265 E4, 285 FCKVAG 290 , E5, 316 SYGGM 320 ) were rabbit antisera. Western blot analysis showed all five interacted positive serum of sheep had been naturally infected SFTSV. Three-dimensional structural modeling BCEs located surface SFTSV-Gn contained flexible loops. sequence alignment revealed high conservation among 13 strains different lineage. These mapped will escalate understanding epitope distribution pathogenic mechanism SFTSV, could provide basis development multi-epitope detection antigen.