Characterizing the cytotoxic effects and several antimicrobial phytocompounds of Argemone mexicana

• DOI: 10.1371/journal.pone.0249704
• Publication Date: 2021-04-07T18:33:08Z
• Authors: Danielle Annette ...; Jeffery Pruet; Clara Patricia Ri...; Estefany Lucia Bo...; Theodore Lefeber; Teodora Najdeska
• Keywords: MTT assay

Commonly called the Mexican prickly poppy, Argemone mexicana is a stress-resistant member of Papaveraceae family plants that has been used in traditional medicine for centuries by indigenous communities Mexico and Western parts United States. This plant exploited to treat wide variety ailments, with reported antimicrobial antioxidant properties, as well cytotoxic effects against some human cancer cell lines. Due its various therapeutic uses abundance secondary metabolites, A. great potential drug discovery candidate. Herein, germination conditions are described activities different (seeds, leaves, inner vs. outer roots) from methanol or hexane extracts preliminarily characterized cells seven unique organisms. When comparing 1 mg each sample normalized background solvent alone, root leaf possessed strongest activity, greatest Gram-positive bacteria tested, less activity Gram-negative fungi tested. Additionally, using MTT colorimetric assay, seed displayed pronounced inhibitory colon cells. Quantification c-MYC (oncogene) APC (tumor suppressor) mRNA levels help elucidate how extract may be affecting After ultra-performance liquid chromatography coupled mass spectrometry subsequent nuclear magnetic resonance analysis fractions, two main antibacterial compounds, chelerythrine berberine, have identified. The roots were found possess both phytocompounds, while lacked chelerythrine. These data highlight importance an invaluable pharmaceutical resource at time when anticancer plateaued.