Complete sequences of epidermin and nukacin encoding plasmids from oral-derived Staphylococcus epidermidis and their antibacterial activity

• DOI: 10.1371/journal.pone.0258283
• Publication Date: 2022-01-18T18:53:54Z
• Authors: Kenta Nakazono; Mi Nguyen-Tra Le; Miki Kawada-Matsuo; Noy Kimheang; Junzo Hisatsune; Yuichi Oogai; Masanobu Nakata; Norifumi Nakamura; Motoyuki Sugai; Hitoshi Komatsuzawa
• Keywords: ORFS

Staphylococcus epidermidis is a commensal bacterium in humans. To persist the bacterial flora of host, some bacteria produce antibacterial factors such as antimicrobial peptides known bacteriocins. In this study, we tried to isolate bacteriocin-producing S . strains. Among 150 isolates from oral cavities 287 volunteers, detected two strains, KSE56 and KSE650. Complete genome sequences strains confirmed that they carried epidermin-harboring plasmid pEpi56 nukacin IVK45-like-harboring pNuk650. The amino acid sequence epidermin was identical previously reported sequence, but synthesis-related genes were partially different. prepeptide KSE650 IVK45 showed one mismatch, both mature entirely similar. pNuk650 larger had an additional seven ORFs compared pIVK45. We then investigated activity against several skin found their different patterns. conclusion, report complete 2 plasmids coding for bacteriocins , which those reported. Furthermore, first show epidermin-carrying plasmid, pEpi56.