A systems radiation biology approach to unravel the role of chronic low-dose-rate gamma-irradiation in inducing premature senescence in endothelial cells

• DOI: 10.1371/journal.pone.0265281
• Publication Date: 2022-03-14T17:44:26Z
• Authors: Gabriele Babini; Giorgio Baiocco; Sofia Barbieri; Jacopo Morini; Traimate Sangsuwan; Siamak Haghdoost; Ramesh Yentrapalli; Omid Azimzadeh; Charlotte Rombouts; An Aerts; Roel Quintens; Teni Ebrahimian; Mohammed Abderraf...; Raghda Ramadan; Sarah Baatout; Soile Tapio; Mats Harms-Ringdahl; Andrea Ottolenghi
• Keywords: Senescence

Purpose The aim of this study was to explore the effects chronic low-dose-rate gamma-radiation at a multi-scale level. specific objective obtain an overall view endothelial cell response, by integrating previously published data on different cellular endpoints and highlighting possible mechanisms underpinning radiation-induced senescence. Materials methods Different datasets were collected regarding experiments human umbilical vein cells (HUVECs) which chronically exposed low dose rates (0, 1.4, 2.1 4.1 mGy/h) gamma-rays until replication arrested. Such analyzed for complementary distinct time points (up several weeks), investigating functions such as proliferation, senescence angiogenic properties, well using transcriptomics proteomics profiling. A mathematical model proposed describe proliferation Results Simultaneous ceasing onset function reproduced logistic growth curve, conveying shared equilibria between two endpoints. combination all investigated highlighted dose-dependence prematurely induced However, molecular appeared be dissimilar rates, thus suggesting more complex scenario. Conclusions This conducted datasets, focusing their temporal dynamics, systems biology approach. our analysis highlight that have in inducing premature senescence, total cumulative absorbed also plays important role accelerating