Epidemiology and molecular characterization of Staphylococcus aureus causing bovine mastitis in water buffaloes from the Hazara division of Khyber Pakhtunkhwa, Pakistan

• DOI: 10.1371/journal.pone.0268152
• Publication Date: 2022-05-05T18:03:40Z
• Authors: Salma Javed; JoAnn McClure; Muhammad Ali Syed; Osahon Obasuyi; Shahzad Ali; Sadia Tabassum; Mohammad Ejaz; Kunyan Zhang
• Keywords: Molecular Epidemiology

Buffalo represent a major source of milk in Pakistan. However, production is impacted by the disease bovine mastitis. Mastitis causes significant economic losses, with Staphylococcus aureus (S. aureus) being one its causative agents. While much work has been done understanding epidemiology mastitis Pakistan, detailed molecular characterization associated S. unavailable. In current study both epidemiological and from Hazara division Pakistan are examined. was isolated 18.41% animals, left quarters more prone to infection (69.6%) than right (30.4%). Sub-clinical (75.31%) prevalent clinical (24.69%), infections evenly distributed amongst eight districts. Molecular revealed that only 19.6% isolates were methicillin-resistant, four strains types identified, including ST9-t7867-MSSA, ST9-MSSA, ST101-t2078-MSSA, ST22-t8934-MRSA-IVa. Antiseptic resistance genes not detected isolates, low levels antibiotic also noted, however methicillin-resistant had higher overall resistance. This represents most complete typing data for causing district country as whole.