Multilocus Sequence Typing helps understand the genetic diversity of Cryptosporidium hominis and Cryptosporidium parvum isolated from Colombian patients

• DOI: 10.1371/journal.pone.0270995
• Publication Date: 2022-07-08T17:44:28Z
• Authors: Johanna Uran-Vela...; Juan F. Alzate; Ana E. Farfan-Garcia; Oscar G. Gomez-Du...; Larry L. Martinez...; Diego D. Domingue...; Winston Rojas; Ana Luz Galvan-Diaz; Gisela M. Garcia-...
• Keywords: Cryptosporidium parvum; Multilocus sequence typing

Multilocus Sequence Typing has become a useful tool for the study of genetic diversity and population structure different organisms. In this study, MLST approach with seven loci ( CP47 , MS5 MS9 MSC6-7 TP14 gp60 ) was used to analyze Cryptosporidium hominis parvum isolated from 28 Colombian patients. Five species were identified: C. felis meleagridis suis . Unilocus analysis identified four allelic families (Ia, Ib, Id, Ie) two (IIa IIc). There polymorphic behavior all markers evaluated both particularly markers. Phylogenetic consensus sequences (CS) showed taxonomic agreement results obtained 18S rRNA gene. Additionally, monophyletic clades that clustered detected, higher number subclades within groups compared those Thirteen MLG eight Haplotypic nucleotide but only latter affected by exclusion CS analysis. The gene fixation index an evolutionary closeness between samples less greater sequence divergence in samples. Data work support implementation considering more detailed information it provides, which may explain some events unilocus could not be established. This is first multilocus intra-specific variability humans South America.