Distribution of human papillomavirus genotypes by severity of cervical lesions in HPV screened positive women from the ESTAMPA study in Latin America

• DOI: 10.1371/journal.pone.0272205
• Publication Date: 2022-07-29T17:35:32Z
• Authors: Rita Mariel Correa; Armando Baena; Joan Valls; María Celeste Col...; Laura Mendoza; Maryluz Rol; Carolina Wiesner; Annabelle Ferrera; María Dolores Fel...; Joaquín Víctor Go...; Jorge Alejandro B...; Pamela Mongelos; Mercedes Rodrigue...; Agustina Saino; Elena Kasamatsu; Carlos Velarde; Ninoska Macavilca; Sandra Martinez; Gino Venegas; Alejandro Calderón; Guillermo Rodriguez; Hernán Barrios; Rolando Herrero; Maribel Almonte; María Alejandra P...
• Keywords: Human papillomavirus; Genotype Distribution; Genotype; Epidemiology; Science; Human Papillomavirus and Cervical Cancer Epidemiology; Uterine Cervical Neoplasms; Microbiology; Gene; 03 medical and health sciences; Vaginal Microbiome and Sexually Transmitted Infections; 0302 clinical medicine; Health Sciences; Genetics; Humans; Papillomaviridae; Internal medicine; Biology; Cancer; Immunology and Microbiology; Human papillomavirus 16; Cervical intraepithelial neoplasia; Hepatitis B Infection and Treatment; Q; Papillomavirus Infections; R; Cohort; Gastroenterology; Life Sciences; Uterine Cervical Dysplasia; Latin America; Oncology; Gynecology; FOS: Biological sciences; Cervical cancer; Medicine; Female; HPV Vaccination; Research Article

The proportion of HPV16 and 18-associated cervical cancer (CC) appears rather constant worldwide (≥70%), but the relative importance other HR-HPV differs slightly by geographical region. Here, we studied HPV genotype distribution positive Latin American (LA) women histological grade, in a sub-cohort from ESTAMPA study; also explored association age-specific genotypes severe lesions. Cervical samples 1,252 participants (854 ≤CIN1, 121 CIN2, 194 CIN3 83 CC) were genotyped two PCRs-Reverse Blotting Hybridization strategies: i) Broad-Spectrum General Primers 5+/6+ ii) PGMY9/11 PCRs. was most frequently found all grades, increased with severity lesions 14.5% ≤ CIN1, 19.8% 51.5% to 65.1% CC (p < 0.001). For remaining HR-HPVs their frequency did not increase when compared less categories. nonavalent vaccine HR-types ranked at top CC, dominant ones being HPV45. single infection occurs, respectively, 57.1% 57.0% ≤CIN1 increasing 72.2% 91.6% (p<0.001). No between age type observed although risk cases age. Results confirm relevance whole clinical spectrum, strong rise its cancer. This information will be relevant evaluating impact vaccination, as baseline against which compare changes type-specific vaccinated participate screening LA Likewise, these data may help select best testing system for HPV-based efficient, affordable, sustainable programmes.