The antiaging effects of a product containing collagen and ascorbic acid: In vitro, ex vivo, and pre-post intervention clinical trial

• DOI: 10.1371/journal.pone.0277188
• Publication Date: 2022-12-12T18:25:31Z
• Authors: Tae Kyeong Ryu; Hanna Lee; Dong Keon Yon; Da Yeong Nam; Soo Yun Lee; Byung Ho Shin; Go Woon Choi; Da Som Jeon; Bo Bae Oh; Ji Hyun Kim; Young Yoon; Hyun Jeong Kim; Luc Duteil; Christelle Bruno-...; Chan Yeong Heo; So Min Kang
• Keywords: 0301 basic medicine; Science; Q; R; Ascorbic Acid; Fibroblasts; Skin Aging; 3. Good health; 03 medical and health sciences; Medicine; Humans; Female; Collagen; Research Article; Skin

Various substances, including collagen (Naticol®) and ascorbic acid, that inhibit and prevent skin aging have been studied. Collagen prevents skin aging, has anti-inflammatory effects, and assists in normal wound healing. Ascorbic acid is a representative antioxidant that plays a role in collagen synthesis. To achieve a synergistic effect of collagen and ascorbic acid on all skin types, we prepared a product named “TEENIALL.” In addition, we used a container to separate ascorbic acid and collagen to prevent the oxidation of ascorbic acid. To confirm the effects of TEENIALL, we first confirmed its penetrability in fibroblasts, keratinocytes, melanocyte, and human skin tissues. Thereafter, we confirmed the collagen synthesis ability in normal human fibroblasts. Based on the results of in vitro tests, we conducted a clinical trial (KCT0006916) on female volunteers, aged 40 to 59 years, with skin wrinkles and hyperpigmentation, to evaluate the effects of the product in improving skin wrinkles, skin lifting, and pigmentation areas before using the product, and after 2 and 4 weeks of using the product. The values of nine wrinkle parameters that were evaluated decreased and those for skin sagging, pigmentation, dermal density, and mechanical imprint (pressure) relief were improved. Skin wrinkle and pigmentation were evaluated to ensure that the improvement effect was maintained even after 1 week of discontinuing the product use. The evaluation confirmed that the effects were sustained compared to those after 4 weeks of using the product. Additionally, skin wrinkles, skin lifting, radiance, and moisture content in the skin improved immediately after using the product once. Based on the results of in vitro and ex vivo experiments and the clinical trial, we show that the product containing ascorbic acid and collagen was effective in alleviating skin aging.